Effects of latrunculin B on the actin cytoskeleton and hyphal growth in Phytophthora infestans.
Identifieur interne : 001547 ( Main/Exploration ); précédent : 001546; suivant : 001548Effects of latrunculin B on the actin cytoskeleton and hyphal growth in Phytophthora infestans.
Auteurs : Tijs Ketelaar [Pays-Bas] ; Harold J G. Meijer ; Marjolein Spiekerman ; Rob Weide ; Francine GoversSource :
- Fungal genetics and biology : FG & B [ 1096-0937 ] ; 2012.
Descripteurs français
- KwdFr :
- Biologie informatique (MeSH), Composés hétérocycliques bicycliques (métabolisme), Cytosquelette d'actine (effets des médicaments et des substances chimiques), Cytosquelette d'actine (métabolisme), Hyphae (croissance et développement), Hyphae (cytologie), Hyphae (effets des médicaments et des substances chimiques), Phytophthora infestans (croissance et développement), Phytophthora infestans (cytologie), Phytophthora infestans (effets des médicaments et des substances chimiques), Phytophthora infestans (génétique), Thiazolidines (métabolisme).
- MESH :
- croissance et développement : Hyphae, Phytophthora infestans.
- cytologie : Hyphae, Phytophthora infestans.
- effets des médicaments et des substances chimiques : Cytosquelette d'actine, Hyphae, Phytophthora infestans.
- génétique : Phytophthora infestans.
- métabolisme : Composés hétérocycliques bicycliques, Cytosquelette d'actine, Thiazolidines.
- Biologie informatique.
English descriptors
- KwdEn :
- Actin Cytoskeleton (drug effects), Actin Cytoskeleton (metabolism), Bridged Bicyclo Compounds, Heterocyclic (metabolism), Computational Biology (MeSH), Hyphae (cytology), Hyphae (drug effects), Hyphae (growth & development), Phytophthora infestans (cytology), Phytophthora infestans (drug effects), Phytophthora infestans (genetics), Phytophthora infestans (growth & development), Thiazolidines (metabolism).
- MESH :
- chemical , metabolism : Bridged Bicyclo Compounds, Heterocyclic, Thiazolidines.
- cytology : Hyphae, Phytophthora infestans.
- drug effects : Actin Cytoskeleton, Hyphae, Phytophthora infestans.
- genetics : Phytophthora infestans.
- growth & development : Hyphae, Phytophthora infestans.
- metabolism : Actin Cytoskeleton.
- Computational Biology.
Abstract
The actin cytoskeleton is conserved in all eukaryotes, but its functions vary among different organisms. In oomycetes, the function of the actin cytoskeleton has received relatively little attention. We have performed a bioinformatics study and show that oomycete actin genes fall within a distinct clade that is divergent from plant, fungal and vertebrate actin genes. To obtain a better understanding of the functions of the actin cytoskeleton in hyphal growth of oomycetes, we studied the actin organization in Phytophthora infestans hyphae and the consequences of treatment with the actin depolymerising drug latrunculin B (latB). This revealed that latB treatment causes a concentration dependent inhibition of colony expansion and aberrant hyphal growth. The most obvious aberrations observed upon treatment with 0.1 μM latB were increased hyphal branching and irregular tube diameters whereas at higher concentrations latB (0.5 and 1 μM) tips of expanding hyphae changed into balloon-like shapes. This aberrant growth correlated with changes in the organization of the actin cytoskeleton. In untreated hyphae, staining with fluorescently tagged phalloidin revealed two populations of actin filaments: long, axially oriented actin filament cables and cortical actin filament plaques. Two hyphal subtypes were recognized, one containing only plaques and the other containing both cables and plaques. In the latter, some hyphae had an apical zone without actin filament plaques. Upon latB treatment, the proportion of hyphae without actin filament cables increased and there were more hyphae with a short apical zone without actin filament plaques. In general, actin filament plaques were more resilient against actin depolymerisation than actin filament cables. Besides disturbing hyphal growth and actin organization, actin depolymerisation also affected the positioning of nuclei. In the presence of latB, the distance between nuclei and the hyphal tip decreased, suggesting that the actin cytoskeleton plays a role in preventing the movement of nuclei towards the hyphal tip.
DOI: 10.1016/j.fgb.2012.09.008
PubMed: 23036581
Affiliations:
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In oomycetes, the function of the actin cytoskeleton has received relatively little attention. We have performed a bioinformatics study and show that oomycete actin genes fall within a distinct clade that is divergent from plant, fungal and vertebrate actin genes. To obtain a better understanding of the functions of the actin cytoskeleton in hyphal growth of oomycetes, we studied the actin organization in Phytophthora infestans hyphae and the consequences of treatment with the actin depolymerising drug latrunculin B (latB). This revealed that latB treatment causes a concentration dependent inhibition of colony expansion and aberrant hyphal growth. The most obvious aberrations observed upon treatment with 0.1 μM latB were increased hyphal branching and irregular tube diameters whereas at higher concentrations latB (0.5 and 1 μM) tips of expanding hyphae changed into balloon-like shapes. This aberrant growth correlated with changes in the organization of the actin cytoskeleton. In untreated hyphae, staining with fluorescently tagged phalloidin revealed two populations of actin filaments: long, axially oriented actin filament cables and cortical actin filament plaques. Two hyphal subtypes were recognized, one containing only plaques and the other containing both cables and plaques. In the latter, some hyphae had an apical zone without actin filament plaques. Upon latB treatment, the proportion of hyphae without actin filament cables increased and there were more hyphae with a short apical zone without actin filament plaques. In general, actin filament plaques were more resilient against actin depolymerisation than actin filament cables. Besides disturbing hyphal growth and actin organization, actin depolymerisation also affected the positioning of nuclei. 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In oomycetes, the function of the actin cytoskeleton has received relatively little attention. We have performed a bioinformatics study and show that oomycete actin genes fall within a distinct clade that is divergent from plant, fungal and vertebrate actin genes. To obtain a better understanding of the functions of the actin cytoskeleton in hyphal growth of oomycetes, we studied the actin organization in Phytophthora infestans hyphae and the consequences of treatment with the actin depolymerising drug latrunculin B (latB). This revealed that latB treatment causes a concentration dependent inhibition of colony expansion and aberrant hyphal growth. The most obvious aberrations observed upon treatment with 0.1 μM latB were increased hyphal branching and irregular tube diameters whereas at higher concentrations latB (0.5 and 1 μM) tips of expanding hyphae changed into balloon-like shapes. This aberrant growth correlated with changes in the organization of the actin cytoskeleton. In untreated hyphae, staining with fluorescently tagged phalloidin revealed two populations of actin filaments: long, axially oriented actin filament cables and cortical actin filament plaques. Two hyphal subtypes were recognized, one containing only plaques and the other containing both cables and plaques. In the latter, some hyphae had an apical zone without actin filament plaques. Upon latB treatment, the proportion of hyphae without actin filament cables increased and there were more hyphae with a short apical zone without actin filament plaques. In general, actin filament plaques were more resilient against actin depolymerisation than actin filament cables. Besides disturbing hyphal growth and actin organization, actin depolymerisation also affected the positioning of nuclei. In the presence of latB, the distance between nuclei and the hyphal tip decreased, suggesting that the actin cytoskeleton plays a role in preventing the movement of nuclei towards the hyphal tip.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ketelaar</LastName><ForeName>Tijs</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory of Cell Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meijer</LastName><ForeName>Harold J G</ForeName><Initials>HJ</Initials></Author><Author ValidYN="Y"><LastName>Spiekerman</LastName><ForeName>Marjolein</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Weide</LastName><ForeName>Rob</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Govers</LastName><ForeName>Francine</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>10</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Fungal Genet Biol</MedlineTA><NlmUniqueID>9607601</NlmUniqueID><ISSNLinking>1087-1845</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019086">Bridged Bicyclo Compounds, Heterocyclic</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D053778">Thiazolidines</NameOfSubstance></Chemical><Chemical><RegistryNumber>LW7U308U7U</RegistryNumber><NameOfSubstance UI="C037068">latrunculin B</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D008841" MajorTopicYN="N">Actin Cytoskeleton</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019086" MajorTopicYN="N">Bridged Bicyclo Compounds, Heterocyclic</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053778" MajorTopicYN="N">Thiazolidines</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>07</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>09</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>09</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>10</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>10</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>4</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23036581</ArticleId><ArticleId IdType="pii">S1087-1845(12)00181-8</ArticleId><ArticleId IdType="doi">10.1016/j.fgb.2012.09.008</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country><region><li>Gueldre (province)</li></region><settlement><li>Wageningue</li></settlement><orgName><li>Université de Wageningue</li></orgName></list><tree><noCountry><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name><name sortKey="Meijer, Harold J G" sort="Meijer, Harold J G" uniqKey="Meijer H" first="Harold J G" last="Meijer">Harold J G. Meijer</name><name sortKey="Spiekerman, Marjolein" sort="Spiekerman, Marjolein" uniqKey="Spiekerman M" first="Marjolein" last="Spiekerman">Marjolein Spiekerman</name><name sortKey="Weide, Rob" sort="Weide, Rob" uniqKey="Weide R" first="Rob" last="Weide">Rob Weide</name></noCountry><country name="Pays-Bas"><region name="Gueldre (province)"><name sortKey="Ketelaar, Tijs" sort="Ketelaar, Tijs" uniqKey="Ketelaar T" first="Tijs" last="Ketelaar">Tijs Ketelaar</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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